Cytochemical Detection of G6PD Deficiency in Women
Abstract
ABSTRACT
Background: G6PD deficiency is transmitted X–chromosomally. Females thus either are homozygous or heterozygous deficient. Heterozygous deficient women have mixed population of RBC, one of the erythrocyte populations is G6PD deficient, and the other has normal G6PD activity
Objectives: To estimate the prevalence of G6PD carrier in Iraqi women.
Methods: The present study included 104 females with age ranged (6-70) years in whom blood cells indices and reticulocyte count were normal. A methemoglubin reduction test followed by methemoglobin elution test (using Nile blue stain) was performed to detect heterozygous females where the proportion of G6PD deficient (ghost) cells varies from case to case. A cytochemical test was used for detection of heterozygous female.
Results: It was shown that (0.96%) were homozygous G6PD deficient females and (6.7%) were heterozygous G6PD deficient females.
Conclusions: The cytochemical assay is much cheaper and reliable test for detection of homo and heterozygous also, it is much cheaper than DNA studies, however, it is time consuming more than 3 hrs and technically difficult to performed to be used in specialist centers.
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