Comparison of Three Diagnostic Methods for Helicobacter pylori
Abstract
ABSTRACT
Background: Carriage of Helicobacter pylori in the human stomach is associated with increased risk of peptic ulcer disease, distal gastric adenocarcinoma and gastric ß-cell mucosa associated lymphoid tissue lymphoma. There were many methods used for the diagnosis of this microorganism
Objective: To compare the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of three different H. pylori diagnostic methods.
Methods: Sixty four antral biopsies were collected and three different H. pylori diagnostic procedures were performed, namely Haematoxilin and Eosin (H&E) stain, Silver stain and rapid urease test
Results: Fifty five of 64 patients (85.9%) tested positive for H. pylori infection by all three diagnostic methods, eight patients (12.5%) were negative infection by all three diagnostic methods. Only one case (1.6%) was positive by silver stain and negative by H&E stain and rapid urease test.
Conclusion: The three different diagnostic methods performed equally well in terms of sensitivity, specificity, PPV and NPV. In cases where the level of H. pylori infection is low, it is recommended that more than one diagnostic method should be utilized to decrease the false negative rate.
Copyright (c) 2012 Iraqi Medical Journal
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