Frequencies of 15 Autosomal Short Tandem Repeats Loci in Arabs Iraqi Population Samples

Abstract

ABSTRACT
Background: Genetic diversity and related calculations which form the basis of forensic
biology and population genetics must be fully integrated in any population. In Iraq, the
major ethnic group is the Arabs; the majority (80%) of them were living mainly in the
middle and south of Iraq. Study of Short Tandem Repeats variation, diversity and data for
Arabs population in Iraq must be known as in variable Arab and regional countries to
understand ancestry, descent, “roots,” heritage, or place of origin in addition of their
usefulness in Forensic work.
Objectives: To establish a reference genetic database of allele frequencies in Arab Iraqi
population to use them in evaluation of DNA profile weigh needed in routine forensic
casework and courts of law.
Methods: The frequencies of 15 Forensic STR loci (D8S1179, D21S11, D7S820,
CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX,
D18S51, D5S818, FGA) included in Amp FlSTR® identifier TM PCR amplification kit
panel from Applied Biosystem were investigated for 200 unrelated randomly selected
Arabs Iraqi individuals.
Results: Allele frequencies, probabilities, power of discrimination, chance exclusion,
random match probability, polymorphism information content and Marker’s observed and
expected heterozygosity (Ho and He, respectively), were calculated as a part of statistical
requirement needed for DNA profiles test for variable forensic applications specially
human identification in the Iraq and near region.
Conclusion: Hardy-Weinberg equilibrium analysis was performed for each locus by the
chi-square test(X)², all tested loci showed no significant deviation from Hardy-Weinberg
expectations.